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Heterogeneity of diffusion tensor imaging measurements of fractional anisotropy and mean diffusivity in normal human hearts in vivo
Journal of Cardiovascular Magnetic Resonance volume 17, Article number: O1 (2015)
Cardiac diffusion tensor imaging (cDTI) by cardiovascular magnetic resonance is becoming more robust for clinical imaging. It has the potential to assess microstructural changes, such as disarray in hypertrophic cardiomyopathy, through measures of fractional anisotropy (FA) and mean diffusivity (MD). However, normal variation in regional and transmural FA and MD is not well described.
Twenty normal subjects were imaged on a 3T Siemens Skyra scanner using a breath hold, mono-polar, diffusion weighted STEAM EPI sequence, as previously described. Imaging was performed in a short axis slice of the mid left ventricle, during the systolic pause, with an optimised protocol (bmain 750, bref 150 s/mm2).
FA was higher in the mesocardium (0.46 ±0.04) than the endocardium (0.40 ±0.04, p≤0.001) and epicardium (0.39 ±0.04, p≤0.001) (figure 1). There was no difference between endocardial and epicardial FA (p=1.0). On regional analysis, the FA in the septum was greater than the lateral wall (0.398 ±0.05 vs 0.439 ±0.03 p=0.04). There was a transmural gradient in MD (epicardium 0.87 ±0.07; mesocardium 0.89 ±0.07; endocardium 0.91 ±0.08 ×10-3 mm2/s) with MD greater in the endocardium than epicardium (p=0.04). With the lateral wall (0.87 ± 0.08×10-3 mm2/s) as the reference, the MD was higher in the anterior wall (0.924 ±0.07×10-3 mm2/s, p=0.016) and septum (0.915 ±0.07×10-3 mm2/s, p=0.028). The signal to noise ratio (SNR) was greater in the mesocardium (14.4 ±2.46) than the endocardium (12.9 ±2.14, p<0.001), and epicardium (12.0 ± 2.35, p<0.001). Regional analysis showed the SNR of the lateral wall (11.5 ±1.46) was lower than the septum (16.0 ±3.43, p<0.001) and anterior wall (14.0 ±3.10, p<0.001) (figure 2). The primary eigenvalue in the mesocardium (1.33 ± 0.08×10-3 mm2/s) was greater than in the endocardium (1.28 ± 0.09×10-3 mm2/s, p=0.001) and epicardium (1.24 ± 0.07×10-3 mm2/s1, p<0.001). Transmural analysis of helical angle (HA) suggested a plateau in the mesocardium, with a reduced gradient.
FA and MD measurements are heterogeneous, varying significantly transmurally and regionally in the normal heart. Contributors to this heterogeneity are many, complex and interactive but may include SNR, variations in cardiac microstructure, partial volume effects and strain. These data indicate that the potential clinical use of FA and MD would require measurement standardisation by myocardial region and layer, unless pathological changes substantially exceed the normal variation identified.
The NIHR Cardiovascular Biomedical Research Unit at the Royal Brompton Hospital & Imperial College London.
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McGill, LA., Scott, A.D., Ferreira, P. et al. Heterogeneity of diffusion tensor imaging measurements of fractional anisotropy and mean diffusivity in normal human hearts in vivo. J Cardiovasc Magn Reson 17, O1 (2015). https://doi.org/10.1186/1532-429X-17-S1-O1
- Cardiovascular Magnetic Resonance
- Fractional Anisotropy
- Diffusion Tensor Imaging
- Hypertrophic Cardiomyopathy
- Mean Diffusivity