- Poster presentation
- Open Access
Cardiac MRI detection of infarct size reduction with hypothermia in porcine ischemia reperfusion injury model
© Dash et al; licensee BioMed Central Ltd. 2015
- Published: 3 February 2015
- Infarct Size
- Ischemia Reperfusion
- Reduce Infarct Size
- Endovascular Cool
- Small Infarct Size
Hypothermia has been shown to provide cardiac protection after a STEMI insult in prior animal and post-hoc human studies. However, the influence of pre-reperfusion temperature on myocardial infarct size is not fully characterized. We employed cardiac MRI to determine the impact of moderate hypothermia (32C) upon infarct size in a porcine ischemia-reperfusion injury (IR) model using an endovascular cooling catheter.
Nineteen (19) adult Yorkshire swine underwent 60-minute ischemia reperfusion (IR) injury (left anterior descending coronary artery) with intravascular cooling administered before reperfusion. The swine were split into 3 groups defined by core temperature before reperfusion: a) 32C (n=6) b) 35C (n=7) and c) 38C (control, n=6). Cardiac MRI (CMR, 3T GE Inc.) was performed at day 0 and 6 days post-IR for delayed gadolinium enhancement scar volumes (DEMRI, d6 only), which were analyzed using CMR42 software (Circle CVI, Inc). Hearts were stained for scar and area at risk (AAR) by TTC. Semi-automatic infarct quantification was achieved using two methods: full width half maxium (FWHM), 5 and 6 standard deviations above mean signal (5SD and 6SD).
Pre-reperfusion hypothermia reduces infarct size compared to 38C. Notably, the small infarct sizes observed in 32C were best assessed using a high threshold method of 6SD, and were largely overestimated by 5SD and FWHM methods. Longer-term studies are required to fully evaluate the impact specific pre-reperfusion temperature reduction upon infarct size and long-term cardiac recovery after STEMI.
NIH/NHLBI K08 (RD) Zoll, Inc.
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