Cardiac MR detects the progression of impaired myocardial perfusion reserve in a mouse model of obesity-related cardiomyopathy
Journal of Cardiovascular Magnetic Resonance volume 17, Article number: P82 (2015)
Obesity has become increasing prevalent in western society and is associated with increased risk of heart failure . Increased body weight is independently associated with impaired myocardial perfusion reserve (MPR), even in the absence of obstructive coronary artery disease . Mouse models can elucidate molecular mechanisms that underlie cardiovascular disease. In this study, we used in vivo cardiac MRI in a mouse model of diet-induced obesity to establish the time course of MPR and we further used ex vivo histological and vascular reactivity studies to elucidate factors underlying the MRI results.
Six week old C57Bl/6 mice fed a high-fat diet (HFD) (n = 9) and age-matched C57Bl/6 mice fed a low-fat diet (Control) (n = 9) were imaged at 7T. Imaging was performed at 6, 12, 18 and 24 weeks post-diet. Mice were anesthetized with 1.25% isoflurane and maintained at 36±1°C during MRI. The MRI protocol included multi-slice cine imaging to assess ejection fraction, left-ventricular (LV) mass, LV wall thickness, and LV volumes, and first-pass imaging at rest and with the vasodilator Regadenoson (0.1 µg/g body weight) to quantify MPR. At 25 weeks post-diet, blood pressure was measured non-invasively. Vascular reactivity of isolated coronary arterioles was assessed using cumulative dose responses to adenosine and acetylcholine in a sub-group of the mice. Histology of the aorta detected the presence or absence of systemic atherosclerosis, and myocardial capillary density was quantified.
HFD mice were obese relative to Control mice at 6 weeks of diet (31.8±5.8g vs. 24.5±5.2g, p<0.05) and their body weight progressively increased up to 24 weeks post-diet (45.5±5.3g vs. 30.6±4.4g, p<0.05). Figure 1 shows examples of first-pass perfusion MRI (A-B) and cine MRI (C-D) from a mouse heart. MPR in HFD mice was reduced at 18 and 24 weeks post-diet (Figure 2A, p<0.05 vs. age-matched control). LV mass was increased in HFD mice at 18 weeks (p<0.05 vs. Control) and it further increased at 24 weeks (Figure 2B, p<0.05 vs. Control, HFD at 18 weeks). LV wall thickness was increased in HFD mice at 18 and 24 weeks post-diet (p<0.05 vs. age-matched Control). Vascular reactivity of the coronary arterioles in response to acetylcholine (Figure 2C) and adenosine (Figure 2D) was reduced in HFD mice (p<0.05 vs. Control). There were no significant differences in volume, ejection fraction, blood pressure and capillary density measurements between the two groups. Histology showed no aortic atherosclerosis in HFD or Control mice.
Using cardiac MR, vascular reactivity, and histological studies, we showed that C57Bl/6 mice fed a HFD for 18-24 weeks have LV hypertrophy and reduced vasodilatory capacity at stress with normal capillary density and no aortic plaque. Future studies using cardiac MR and gene-modified mice fed a HFD may shed light on key molecular mechanisms that underlie myocardial ischemia in obesity related cardiomyopathy.
This work was funded by NIH R01 EB001763.
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Naresh, N.K., Butcher, J.T., Chen, X. et al. Cardiac MR detects the progression of impaired myocardial perfusion reserve in a mouse model of obesity-related cardiomyopathy. J Cardiovasc Magn Reson 17 (Suppl 1), P82 (2015). https://doi.org/10.1186/1532-429X-17-S1-P82