- Walking poster presentation
- Open Access
Whole-heart, free-breathing, three-dimensional myocardial BOLD MRI at 3T with simultaneous 13N-ammonia PET in canines
© Yang et al; licensee BioMed Central Ltd. 2015
- Published: 3 February 2015
- Free Breathing
- Adenosine Stress
- Prep Pulse
- Coil Bias
- Poor Spatial Coverage
Myocardial BOLD MRI is an emerging non-contrast approach for the assessment of ischemic heart disease. However, current BOLD CMR methods (T2-weighted, T2*-weighted or bSSFP) are limited by poor spatial coverage and image artifacts (e.g. coil bias, B1 and B0 inhomogeneities). To address these limitations, we developed a fast, free breathing 3D T2 mapping technique at 3T that utilizes near 100% imaging efficiency. This quantitative BOLD approach, which can be performed within 5 minutes, permits full LV coverage during adenosine stress. In this study, we tested our approach in a canine model and validated our findings with simultaneously acquired 13N-ammonia PET perfusion data in a clinical PET-MR system.
Mongrel dogs (n=3) were studied with a state of the art PET-MR system (Biograph mMR, Siemens Healthcare, Germany). After scouting and whole-heart shimming, fast 3D free-breathing T2 mapping sequence were prescribed at rest and under adenosine stress (140 mg/min/kg) with the following scan parameters: TR/TE =3.4/1.7 ms, flip angle = 15°, imaging resolution = 2 x 2 x 6 mm3 with 14 partitions, adiabatic T2 prep pulses (with T2 durations of 0, 24 and 55 ms) every other heart beat. Total acquisition time for whole LV coverage was 5 mins. For validation, dynamic 13N-ammonia PET scans were acquired along with MR data. PET images were analyzed using qPET (Cedars-Sinai Medical Center, US). Epi and endo-cardial contours were used to segment the myocardium and mean myocardial T2 (T2avg) values were measured from basal, mid and apical slices at rest and adenosine stress. The corresponding slices were matched to 13N-ammonia PET images and mean myocardial perfusion (Qavg) values were measured. Myocardial BOLD reserve (T2avg (stress):T2avg(rest)) and perfusion reserve (T2avg (stress):T2avg(rest)) were computed on a slice basis and regressed.
The proposed fast 3D free breathing T2 mapping at 3T permits whole LV assessment of BOLD changes between rest and adenosine stress. The BOLD responses were very closely correlated with PET perfusion, suggesting that the proposed BOLD CMR method is a viable approach for imaging myocardial perfusion. Further studies are required to examine its utility in the setting of ischemic heart disease.
This work was supported in part by National Heart, Lung, and Blood Institute HL091989.The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Heart, Lung, And Blood Institute or the National Institutes of Health.
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