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Apparent Diffusion coefficient (ADC), T1 and T2 quantitative indexes of the myocardium in athletes before, during and after extreme mountain ultra-marathon: correlation with myocardial damages and inflammation biomarkers
Journal of Cardiovascular Magnetic Resonance volume 18, Article number: O41 (2016)
Background
Previous MRI and US studies have shown the existence of functional and biochemical alterations in the myocardium after prolonged endurance exercise, demonstrating transient diastolic dysfunction [1]. Simultaneous transient increases of cTnT and NT-proBNP biomarkers have been reported [2, 3] without focal necrosis identified by delayed enhancement imaging, probably due to a cytosolic's dropping of biomarkers rather than destruction of myocytes. Inflammation, microstructural & functional modifications caused by extreme loading conditions, have never been explored using quantitative MRI.
Methods
We prospectively studied 50 runners enrolled on the 2014 « Tor des Géants » edition (the most extreme mountain ultra-marathon (336 km length, 24000 m cumulative elevation), without clinical evidence of personal history of cardiac or pulmonary disease. Subjects were studied before, at arrival, and after 3 days recovery. Imaging protocol included global and regional LV function analysis and quantitative MRI : T1, T2 and ADC values were obtained using respectively a MOLLI sequence, a radial multi-echo sequence, a Stejskal-Tanner diffusion sequence [4]. T1, T2 and ADC values at 1.5T were compared with plasma levels of inflammation, myocardial stress and/or damage biomarkers including hs-TnT, NT-proBNP, Gal-3 (a carbohydrate binding lectin produced by macrophages, upregulated in hypertrophied heart, emerging as a mediator for fibrosis development and remodeling) and ST2 (a family member of IL-1 receptors known for its role in immunological processes, having a potential role in cardiac pathogenesis).
Results
27 finishers (54%) completed the longitudinal study.T2, T1 and ADC values significantly increased immediately after the race. ADC quickly normalized after recovery while T1, T2 markers remained higher than baseline (Figure 1). Significant correlations were found between myocardial MR biomarkers and blood (Gal3,ST2,NT-proBNP), plasmatic (CRP, CKs, hs-TNT) and cellular (WBC, lymphocytes, neutrophilis) ones (Figure 2).
T1, T2 and ADC sample distributions before, immediately and after 3-days recovery.
Univariate analysis. ADC values vs blood biomarkers. Significant correlations (p-values <0.05) are highlighted with colored overlay. FC = cardiac frequency, HCT = hematocrit, WBC = white blood cell, HFAPB= heart failure acid binding protein, CRP = c-reactive protein.
Conclusions
It is the first study investigating the role of quantitative MR diffusion to explore human acute stress in humans together. ADC, T1 and T2 were all able to identify changes in subjects and related to several plasmatic biomarkers and therefore appear as valuable MR biomarkers of myocardial inflammation at least for this specific type of acute stress. Prior to a deeper understanding of the impact of ultra-endurance, this study hightlights an added value of ADC, that differ from T1 and T2 markers, to scrutinize acute stress phenomena in the myocardium. ADC represents a novel information, revealing more about water redistribution leading to ultraexercise-induced reversible myocardial inflammation. Overal it illustrates the usefulness and complementary nature of ADC as an emerging cardiac biomarker, foreseen to be deployed at short-term in the evaluation of innovative therapeutic strategies targeting inflammation.
References
- 1.
Nottin : 2009
- 2.
Trivax : 1985
- 3.
Hansen : 2011
- 4.
Moulin : 2015
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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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Viallon, M., Moulin, K., Goff, C.L. et al. Apparent Diffusion coefficient (ADC), T1 and T2 quantitative indexes of the myocardium in athletes before, during and after extreme mountain ultra-marathon: correlation with myocardial damages and inflammation biomarkers. J Cardiovasc Magn Reson 18, O41 (2016). https://doi.org/10.1186/1532-429X-18-S1-O41
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Keywords
- Apparent Diffusion Coefficient
- Myocardial Inflammation
- Acute Stress
- Delay Enhancement Imaging
- Innovative Therapeutic Strategy
