- Poster presentation
- Open Access
Flow imaging in vivo using off resonance spin labeling induced by extraneous contrast agent
© Bastiaansen et al. 2016
- Published: 27 January 2016
- Positive Contrast
- Excitation Angle
- Radio Frequency Excitation
- Superparamagnetic Contrast Agent
- Narrowband Image
Tissues within the dipolar field of superparamagnetic contrast agents experience a frequency shift that enables positive contrast MRI with acquisition schemes using on-resonant saturation (Figure 1a,b). The administration of iron oxide based agents enables a plethora of applications using positive contrast MRI. They have been widely explored and mainly focused on lymphography, angiography, cancer detection and atherosclerosis. In this preliminary in vivo study, a dynamic contrast mechanism is presented,using externally placed suspensions of iron oxide nanoparticles (SPIONs) to induce noninvasive spin tagging of nearby blood flow.
A cylindrical tube containing a concentrated suspension of 19 nm SPIONs was placed alongside the upper leg in human volunteers (Figure 1c), causing a local magnetic field change in the adjacent tissue. Protons residing in the off-resonance field experience a frequency shift as they move through or are static within this field. A 3D gradient echo based MRI acquisition, preceded by a narrow or broad bandwidth (100 Hz or 2 kHz) on-resonant saturation pulse (Figure 1d), was used for data acquisition on a 3T clinical MRI scanner. This led to a selective excitation of the off resonance area (Figure 1c). A variable radio frequency excitation angle and delay time ranging from 0 to 0.8 s facilitated the tagging of the off-resonant flowing spins in the nearby veins and arteries. Flowing spins remain unaffected by the narrowband RF pulse and are tagged by the external SPIONs.
Flow imaging with iron induced spin tagging (FLIRT) was achieved using external SPIONs which targeted the positive labeling of moving protons. The feasibility of this method was shown in vivo with preliminary data obtained in human volunteers. It allows for flow visualization in the vicinity of ferrimagnetic objects without contrast injection.
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