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High spatiotemporal resolution hyperpolarized 13C angiography
© Reed et al. 2016
- Published: 27 January 2016
- Magnetic Resonance Angiography
- Dynamic Nuclear Polarization
- Axial Projection
- High Spatiotemporal Resolution
- Angle Ramp
Sub-millimeter resolution, background-free magnetic resonance angiography (MRA) has been performed previously using dynamic nuclear polarization (DNP)- enhanced 13C labeled small molecules [1-4]. This approach of contrast-enhanced MRA is appealing since many of the commonly used DNP substrates are endogenous and could be potentially used in large doses in patients with renal insufficiency. The aim of this study was to combine high spatial resolution MRA with a high frame rate spiral readout , and to test the feasibility of 13C magnetic resonance fluoroscopy in rats on a clinical imaging system.
Male Sprague Dawley rats were anesthetized with a 1% isofluorane / O2 mixture and placed in a dual-tuned 1H/13C birdcage transceiver inside a clinical GE 3T scanner. [13C,15N]urea was polarized on an Oxford Instruments HyperSense, dissolved in a saline / phosphate buffer solution, and administered via lateral tail vein catheters.
Real time scan control and image reconstruction was implemented on the RTHawk platform . An SSFP sequence was tailored for transient phase hyperpolarized imaging by using a 10-step Kaiser-Bessel flip angle ramp with a terminal flip angle of 180 degrees. A variable density spiral readout (12 cm FOV at k = 0, 8 cm FOV at kmax, 1 mm resolution) was utilized for the maximization of signal detection time (8 ms) within a TR (13 ms). The 30 interleaves were gridded, density compensated, and a single image was reconstructed for every 2 TRs giving an effective 26 ms frame rate. Images were acquired as coronal and axial projections.
This study showed preliminary results of high spatial (~1 mm) and high temporal (~30 ms) resolution acquisition of hyperpolarized 13C substrates. Real time 13C angiography could address the potential risks of radiation exposure from X-ray fluoroscopy and nephrotoxicity inherent to most common MRI and CT contrast agents. Image quality will likely improve with the use of multi-frequency reconstruction, parallel imaging, and flow-refocusing gradient pulses.
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