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In vivo multi-modality tracking of the regenerative effects of the human induced pluripotent stem cell-derived cardiomyocytes (iCMs)
Journal of Cardiovascular Magnetic Resonance volume 17, Article number: Q119 (2015)
In vivo multi-modality cellular and molecular imaging of the engrafted iCMs is necessary to characterize the engraftment and the regional effects on the viability of the injured myocardium. Zinc finger nuclease (ZFN)-mediated integration of the reporter gene into the AAVS1 locus in the iCMs and manganese enhanced MRI (MEMRI) should allow precise in vivo detection of myocardial regeneration.
In order to have multi-modal in vivo imaging of the stem cells, ZFN technology was employed to integrate a triple fusion RG (firefly luciferase (BLI), red fluorescent protein (RFP), and herpes simplex virus thymidine kinase (PET)) into the AAVS1 locus of iPSCs. iPSCs monoclonal lines were then differentiated into iCMs, under chemically defined conditions using CHIR (4 µM) and C59 (2 µM) small molecules to modulate the Wnt pathway activity. The produced iCMs were then fully characterized and injected into mice and tracked with several techniques.
Co-localization of cardiac phenotype and striated morphology, flow cytometry measurement of 93% cardiac troponin (cTNT) associated with 75% VCAM and 49% CIRPA cell surface markers, the atomic force measurement with calcium flux were observed, which confirmed normal function of the iCMs (Fig. 1A). The iCMs were, then, transplanted into murine myocardial injury model. Two and four weeks post-injury, left ventricular ejection fraction (LVEF) was better preserved in the iCM-treated vs. PBS-treated mice (week 2: 33.1±4.4% vs. 26.1±7.9%, p<0.05 and at week 4: 35.1±8.4% vs. 16.1±0.2%, p<0.05). MEMRI also demonstrated increased % myocardial viability by iCMs at week 2 measured 67.3±13.1%, which decreased slightly by -2.3±0.3% at week 4 while PBS treated cases conferred significant decrease -17.4% at week 4 (p<0.05). Finally, the mean BLI signal, indicating iCM engraftment, demonstrated on days 14 (n=3) is 2.1±0.6 x103p/s/cm2/sr and days 28 (n=3) is 4.2±2.2 x103p/s/cm2/sr. Robust in vivo expression of ZFN-edited BLI signal of iCM engraftment and sustained MEMRI signal of myocardial viability over 4-week duration demonstrated regeneration of the injured myocardium (Figs. 1B and 1C).
This study demonstrates the successful use of in vivo multi-modality imaging to confirm the regeneration of the injured myocardium. ZFN technology enables targeted site-specific insertion of RGs and MEMRI allows direct evaluation of myocardial viability. These imaging capabilities will allow clinical translation of iCMs.
NIH/NHLBI UM1 (HL-12-026).
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Mahmoudi, M., Rulifson, E., Tachibana, A. et al. In vivo multi-modality tracking of the regenerative effects of the human induced pluripotent stem cell-derived cardiomyocytes (iCMs). J Cardiovasc Magn Reson 17 (Suppl 1), Q119 (2015). https://doi.org/10.1186/1532-429X-17-S1-Q119
- Thymidine Kinase
- Myocardial Viability
- Zinc Finger Nuclease
- Herpes Simplex Virus Thymidine Kinase
- Injured Myocardium