- Oral presentation
- Open Access
An isolated pig heart for the development, validation and translation of novel magnetic resonance techniques
Journal of Cardiovascular Magnetic Resonance volume 12, Article number: O17 (2010)
Novel magnetic resonance (MR) techniques and imaging biomarkers are often validated in small animal models or empirically in patients. The direct translation of small animal cardiac MR imaging protocols to humans is rarely possible, while validation of novel imaging techniques in humans by tracking changes in MR biomarkers in response to externally controlled changes in blood flow, for example, is difficult, or unethical. An isolated blood-perfused pig heart model which closely resembles human physiology, anatomy and size, can be exquisitely controlled in terms of regional blood flow, oxygenation, afterload and workload, and can be imaged by the same equipment used for humans. It would therefore be a valuable tool for the development, validation and translation of novel magnetic resonance techniques.
To design and build a novel MR-compatible, explanted, blood-perfused and free-beating pig heart model and test its feasibility at a clinical 3 Tesla MR Scanner.
We have designed and built a fully MR-compatible, free beating, blood-perfused, isolated pig heart preparation, capable of being run in an isolated coronary perfusion mode and in working heart mode, providing control of numerous physiologic parameters (see table 1 and figure 1). Hearts were explanted from Large White Cross Landrace pigs (average weight of 50 kg, Harlan Laboratories UK) under terminal anaesthesia and transported to the laboratory under cold cardioplegic arrest (STH solution). The perfusion system consists of a separate haemoperfusate and dialysate circuit. Blood temperature and oxygenation is controlled by an oxygenator with an integrated heat exchanger.
We tested functional cardiac imaging (CINE), high-resolution perfusion imaging (<2 × 2 mm) using a combination of 3 Tesla, parallel imaging (SENSE) and temporal undersampling (kt), as well as late gadolinium enhancement imaging.
The isolated pig heart preparation can be run in the MR environment, with stable physiological function for approximately 4 hours. Image quality was comparable to clinical imaging. Myocardial perfusion imaging was performed at different flow rates including selective intracoronary gadolinium injections. Late gadolinium enhancement imaging was performed in a model of acute myocardial infarction (see figure 2).
The technical design of an isolated pig heart model allows to represent and image in situ cardiac function ex vivo. This novel system allows for excellent control of physiological parameters, validation against gold standards, and easy translation of the methods to patients using the same equipment and imaging sequences.
About this article
Cite this article
Schuster, A., Chiribiri, A., Southworth, R. et al. An isolated pig heart for the development, validation and translation of novel magnetic resonance techniques. J Cardiovasc Magn Reson 12, O17 (2010). https://doi.org/10.1186/1532-429X-12-S1-O17
- Cardiac Magnetic Resonance
- Myocardial Perfusion Imaging
- Late Gadolinium Enhancement Imaging
- Magnetic Resonance Technique
- Tesla Magnetic Resonance