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Effects of steroids and angiotensin converting enzyme inhibition on circumferential strain in boys with Duchenne muscular dystrophy: a cross-sectional and longitudinal study utilizing cardiovascular magnetic resonance



Steroid use has prolonged ambulation in Duchenne muscular dystrophy (DMD) and combined with advances in respiratory care overall management has improved such that cardiac manifestations have become the major cause of death. Unfortunately, there is no consensus for DMD-associated cardiac disease management. Our purpose was to assess effects of steroid use alone or in combination with angiotensin converting enzyme inhibitors (ACEI) or angiotension receptor blocker (ARB) on cardiovascular magnetic resonance (CMR) derived circumferential strain (εcc).


We used CMR to assess effects of corticosteroids alone (Group A) or in combination with ACEI or ARB (Group B) on heart rate (HR), left ventricular ejection fraction (LVEF), mass (LVM), end diastolic volume (LVEDV) and circumferential strain (εcc) in a cohort of 171 DMD patients >5 years of age. Treatment decisions were made independently by physicians at both our institution and referral centers and not based on CMR results.


Patients in Group A (114 studies) were younger than those in Group B (92 studies)(10 ± 2.4 vs. 12.4 ± 3.2 years, p < 0.0001), but HR, LVEF, LVEDV and LVM were not different. Although εcc magnitude was lower in Group B than Group A (-13.8 ± 1.9 vs. -12.8 ± 2.0, p = 0.0004), age correction using covariance analysis eliminated this effect. In a subset of patients who underwent serial CMR exams with an inter-study time of ~15 months, εcc worsened regardless of treatment group.


These results support the need for prospective clinical trials to identify more effective treatment regimens for DMD associated cardiac disease.


Duchenne muscular dystrophy (DMD) is an X-linked recessive disorder affecting approximately 1:3,500 males [13]. Boys with DMD are currently treated with corticosteroids at a young age to prolong ambulation. This therapy combined with improvements in respiratory care have resulted in increased survival [46] such that DMD-associated heart disease is now the leading cause of mortality [711]. Myocardial changes, as a result of the lack of dystrophin, consist of cell membrane degradation, interstitial inflammation, edema, fatty replacement and fibrosis [1215]. Despite reports in small cohorts of the beneficial cardiovascular effects of corticosteroids [6, 16] and angiotensin converting enzyme inhibitors (ACEI)/angiotensin receptor blockers (ARB) [1719], there is no consensus regarding the management of DMD-associated cardiac disease. In the absence of conclusive randomized clinical trial data, steroids, ACEI, ARB, beta blockers (BB) and/or digoxin have been used empirically [20, 21]; beneficial effects of positive inotropic agents have been reported in boys with depressed left ventricular function and advanced cardiac disease [22].

Defining clinical endpoints for cardiac therapy in DMD boys is challenging. Recent studies from our center and others have shown that indices of global left ventricular (LV) function, e.g. mass, volume and ejection fraction (EF) are not adequate to detect cardiac dysfunction in young DMD patients [2325], but myocardial strain (ε), an indicator of local myocardial deformation normalized to its original dimension, can detect occult cardiac disease early in the course of DMD despite normal EF. Further, depressed εcc magnitude correlates with disease progression [24, 25].

The purpose of this study was to retrospectively compare cardiac effects of corticosteroid monotherapy versus corticosteroids plus ACEI or ARB in a cohort of DMD patients followed at our center. We compared effects on both global LV function (EF) and local LV function (εcc) determined by cardiovascular magnetic resonance (CMR).


DMD patients >5 years of age who underwent CMR from February 2006 to February 2010 were identified from the CMR database at Cincinnati Children's Hospital Medical Center (CCHMC). The diagnosis of DMD was confirmed by physical examination and identification of a dystrophin mutation. This study was approved by the Institutional Review Board.

Patients were identified for inclusion in one of two treatment groups. Group A was only treated with corticosteroids (either deflazacort or prednisone). Group B was being treated with corticosteroids plus ACEI (lisinopril or enalapril) or ARB (losartan). All patients in Group B had been treated with ACEI/ARB for a minimum of 12 months prior to CMR, and all patients in both groups had been treated with corticosteroids for at least 12 months prior to CMR. Initiation of corticosteroids and ACEI/ARB was determined exclusively by treating physician preference and were not based on CMR results. DMD boys not treated with corticosteroids or treated with beta blockers were excluded.

CMR studies were conducted on either a 3 Tesla (Trio, Siemens Medical Solutions, Erlangen, Germany) or 1.5 Tesla (Signa Excite, General Electric Healthcare, Milwaukee, WI) scanner based on clinical availability, independent of the patient's clinical status or prior type or field strength of the first study. Cardiac functional imaging was performed using retrospective ECG-gating, segmented Steady State Free Precession (SSFP) technique after localized shimming and/or frequency adjusting. Subjects were breath-held as tolerated; for those subjects who could not adequately breath-hold, a free breathing technique with multiple signal averaging was used. Standard imaging included a short axis stack of cine SSFP images from cardiac base to apex; the short axis was prescribed as the perpendicular plane to the left ventricular long axis in 2 and 4 chamber views as previously described [26, 27]. Typical scan parameters included FOV = 32-38 cm, slice thickness = 5-6 mm, gap = 1-2 mm, NEX = 2 (breath hold; 4-5 for free breathing), TE/TR = 1.4/2.8 (Siemens), TE/TR = 2.0/4.0 (GE), in-plane resolution = 1.2- 2.2 mm. A minimum of 12 slices were performed, with 20 phases/slice. The typical temporal resolution of the cine SSFP images was 30-40 ms; they and were adjusted according to the patient heart rate and ability to breath-hold. The RF flip angles were set between 50°-70° dependent on the patient weight, height and the SAR level.

Tagged cine CMR images were acquired in the short axis of the midventricle at the level of the papillary muscles using an ECG-triggered segmented k-space fast gradient echo sequence with spatial modulation of magnetization in orthogonal planes. Tag imaging was performed prior to administration of Gadolinium. Grid tag spacing was 7-8 mm. The scan parameters used were: field of view = (30-32) × (25-26) cm2, slice thickness = 6 mm, flip = 20°, TE/TR = 3 ms/6.6 ms (GE), = 3 ms/4.2 ms (Siemens), views per segment = 8 (GE), = 7-9 (Siemens). Tagged images were analyzed using the HARmonic Phase (HARP, Diagnosoft, CA, USA) technique [23, 2832]. Only the mid-ventricular slice was analyzed, based on our experience and others' [23] of limited reproducibility of the basal and apical slices. Details of the εcc analysis have been previously described by Hor et al. [24]. The εcc data was exported to a spreadsheet file for analysis.

Cardiac functional imaging was performed to determine LV volumes, mass and EF using semi-automated endocardial and epicardial segmentation (QMASS v.6.1.5, Medis Medical Imaging Systems) Netherlands and circumferential strain (εcc) using HARmonic Phase analysis (HARP, Diagnosoft, Palo Alto, California) as previously described [2325, 2931, 3335].

Results are expressed as mean ± SD for continuous data and as percentages and numbers for categorical data. Continuous variables were compared using two-sample t-test and categorical variables were compared using Fisher exact-test. Analysis of covariance (ANCOVA) was used to assess the effect of medications on several clinical measurements with age as a continuous covariate in the model. All tests were 2-sided, and a p-value < 0.05 was considered statistically significant. SAS version 9.2 (SAS Institute Inc., Cary, NC) was used for all analyses.


We identified 171 DMD patients > 5 years of age that had undergone CMR examination. A total of 256 studies were available for review as a number of patients had multiple studies. We excluded 50 studies for either no corticosteroid treatment at the time of study (36 studies) or treatment with beta-blockers at the time of study (14 studies). The remaining 206 studies from 136 patients were divided into two groups: Group A (corticosteroids alone, n = 114) and Group B (corticosteroids plus ACEI or ARB, n = 92). Group A patients were significantly (p < 0.0001) younger (10 ± 2.4 years) than Group B patients (12.4 ± 3.2 years).

The average steroid dose was similar between Group A and Group B (0.7 ± 0.29 mg/kg/day, range 0.1-1.9 vs. 0.6 ± 0.22 mg/kg/day, range 0.1 - 1.3, p = 0.48). The patients in Group B were on standard dose of ACEI (0.16 ± 0.29 mg/kg/day, range 0.04 - 1.23) and ARB (0.73 ± 0.29 mg/kg/day, range 0.34 - 1.23) (Table 1).

Table 1 DMD Patients characteristics

Heart rate, LV EF, LV end diastolic volume (LVEDV) and LV mass (LVM) were not different between the two groups. LVEF averaged across all patients in this cohort was normal, but εcc magnitude was significantly lower in Group B (-12.8 ± 2) versus Group A patients (-13.8 ± 1.9, p = 0.0004). To determine the extent to which differences in the two groups was an effect of patient age, we performed a covariance analysis and treated age as a continuous variable (Table 2). The covariance model confirmed the lack of significant differences between Groups A and B with respect to heart rate, EF, LVEDV, LVM, LVRI or εcc after controlling for age, implying lack of difference between the two groups other than the use of cardiac medications in Group B.

Table 2 Analysis of covariance summary results: Comparisons between steroid only vs. steroid plus ACEI_ARB (medication) adjusted for age as a continuous variable

Additionally, to assess serial changes in EF and εcc we identified patients in Group A (n = 28) and B (n = 31) who underwent 2 CMR studies during the observation period. This included 11 patients who crossed from Group A to B between sequential studies. Among individuals with 2 studies, EF and εcc values were compared between the two studies (Figure 1). EF changes between the first and second study were variable across three groups, while the majority of patients had decline in εcc magnitude over the same study period. In Group A, 50% (14/28) patients had decrease EF while the other 50% (14/28) had increase EF. Of Group A patients, 7% (2/28) had changes in EF by > 10%, however one increased by almost 15% and the other decrease by 12%. Both patient s continue to have normal EF (>55%) on both studies. One patient had EF < 55% on the first study which remained the same on the second study and another patient had EF that decline from 59% to 54%. However, only 14% (4/28) of Group A subjects had an increase εcc magnitude while 86% (24/28) had worsening of εcc magnitude. The findings for Group B were similar, with 68% (21/31) of patients having decrease EF and 32% (10/31) of patients with increase EF over the two study. Of Group B patients, 28% (9/32) had changes in EF by > 10% with 6 patients having decrease EF and 3 patients with increase EF on the follow-up study. Of these, One patient started with an EF of 53% and increased to 64% on the follow-up study. Of the 6 patients with decrease in EF, three had EF < 55% while the other three continue to have normal EF on the follow-up study. As in Group A, 84% (26/31) of the Group B patients had decrease εcc, while only 16% (5/31) subjects showed improvement in εcc magnitude. For the patients that crossed from Group A to B, the percent of patients with improved EF was 45% (5/11) while 55% (6/11) had decline in EF. The percent of patients with an increase in εcc magnitude was 18% (2/11) but 82% (9/11) had worsening of εcc magnitude (Figure 1). The average time between studies was the same across Group A, Group B and patients who transitioned from Group A to B. LVEF did not change significantly between baseline and follow-up in all three groups (Table 3). While εcc worsened from initial to follow-up CMR exam in all three groups, reduction in εcc only reached statistical significance in Group B (Table 3).

Figure 1
figure 1

Serial ejection fraction (EF) and circumferential strain (ε cc ). Red, indicates decreased EF and εcc magnitude; blue, indicates increase EF and εcc magnitude and black, indicates mean EF and εcc values. Panels A-C show EF of each subject plotted serially; there was no significant change in mean EF across all three groups. Individually 14/28 (50%) in Group A, 21/31(68%) in Group B and 5/11 (45%) in Group A to B transition had decrease EF, while 14/28 (50%), 10/31 (32%) and 6/11 (55%) of the individuals in the respective groups had increase EF. Panels D-F show εcc of each subject plotted serially; although all three groups had lower absolute εcc on the follow-up study, only Group B subjects had a significant decline (p = 0.007). Individually εcc magnitude decreased more consistently across all three groups, with 24/28 (86%) in Group A, 26/31 (84%) in Group B and 9/11 (82%) in Group A to B. Unlike EF, only a small fraction of the individuals have improvement of εcc magnitude on follow-up study with 4/28 (14%), 5/31 (31%) and 2/11 (18%) of the individuals in the respective group having an increase εcc value on follow-up study.

Table 3 Results from Serial CMR Exams Across DMD Treatment Groups


This study represents the largest cohort of DMD patients analyzed for the effect of medical therapy on the natural history of DMD-associated cardiac disease. Albeit retrospective, these results suggest that treatment with either an ACEI or ARB does not arrest the decline in DMD-associated cardiac function in patients receiving corticosteroids. In fact, we found a statistically significant worsening in εcc in patients taking both corticosteroids and ACEI or ARB, suggesting that current approaches are inadequate in arresting decline in cardiac function.

Clinical assessment of DMD-associated cardiac disease is particularly challenging, as overt decompensation typically occurs only in the terminal stages of disease at a time when clinical assessment of cardiac function is limited. A sensitive and specific parameter such as εcc could thus assume great importance in management of these patients. Previous studies have shown that changes in εcc precede decline in LVEF in DMD-associated cardiac disease as well as in both hypertrophic and hypertensive cardiomyopathy [24, 3638]. Using such an early marker of subclinical disease in a cohort with preserved LVEF, we were unable to detect an improvement with standard therapies.

Assessment of cardiac effects of corticosteroid therapy in animal models and patients has historically yielded mixed results. For example, corticosteroids have been shown to improve systolic function in children with myocarditis but not with dilated cardiomyopathy [39]. Markham et al demonstrated that freedom from ventricular dysfunction at approximately 50 months between retrospectively-reviewed echocardiograms was 93% for steroid treated versus 53% for untreated DMD cases [6]. However, in a mouse model of dystrophin deficiency, Bauer et al demonstrated acceleration of cardiac dysfunction in steroid treated animals [40]. Recently, Pereira et al [41] observed normalization of multidirectional (radial, longitudinal and circumferential) strain assessed by 2D echo speckle tracking in Cushing syndrome patients following normalization of corticosteroid excess; they concluded that corticosteroid excess not only induced LV hypertrophy and diastolic dysfunction but also subclinical systolic dysfunction, which reverses upon normalization of corticosteroid excess. While there is universal agreement that steroids prolong ambulation in the DMD patient population (reviewed in [21]), the current study failed to identify a cardioprotective effect of steroids.

Several studies have suggested beneficial effects of ACEI on LV function in small cohorts [1719, 42]. In a murine DMD model, Bauer et al showed hemodynamic benefit to dystrophin-deficient mice treated with ACEI alone [40]. Jefferies et al suggested that this effect may be linked to specific mutations [19] in humans, but Ramaciotti et al [42] did not confirm this finding. In addition to εcc, we found no difference in EF between baseline and 15 months, which is at odds with the findings of Duboc et al [17] who found that treatment with perindopril delayed the onset and progression of LV dysfunction in children. There are several possible explanations for the differences in findings. First, in our cohort, the time between the serial CMR studies might be insufficient to develop detectable LV EF decline. However, one would expect to detect more subclinical changes using the more sensitive measure LV εcc. Furthermore, without an untreated control group, the fact that εcc magnitude declined only slightly may represent a "good" outcome, as LV function of untreated patients may decline more precipitously. Despite smaller studies reporting benefit of ACEI and BB, there is no published data demonstrating longer life expectancy in DMD boys undergoing "adult-like" CHF therapy.

A long-standing hypothesis regarding DMD-associated cardiac disease pathogenesis is that loss of membrane integrity is a primary event leading to myocyte degeneration. Intermittent tears in the cell membrane permit influx of calcium that then functions as a primary inducer of a destructive cascade culminating in myocyte necrosis and replacement fibrosis [43]. Although there has been intense interest in treating DMD-associated cardiac disease, successful therapies remain elusive [22, 4446]. Findings in the current study underscore the need for randomized studies in DMD population. As in cases of heart failure in adult population, development of heart failure with normal EF starts early (HFNEF) and proceeds to systolic dysfunction. Of interest, studies in adult HFNEF patients treated with ACEI or BB have not demonstrated improvement in mortality compared to placebo [4749]. In boys with DMD, dystrophin mutation dictates eventual LV dysfunction and once systolic dysfunction and myocardial fibrosis develop, clinical disease typically progresses very rapidly and long-term survival (as seen in adult patients with LV systolic dysfunction) is poor. As such, the window of opportunity to treat DMD-associated cardiac disease needs to be advanced to when εcc is abnormal but EF has not yet begun its inexorable declined.

Study Limitations

Study design based on physician preference is a major limitation of the study and includes retrospective, mostly cross-sectional data with no control group and with strong confounding of group allocation by age of boys being prescribed therapy. Older boys tended to be on combination treatment while younger boys were often treated with steroids alone. Further, longitudinal data is limited. Other confounding factors are very difficult to control for but are beyond the scope of this manuscript. As such, no firm conclusions can be drawn regarding the response rates to steroid or ACEI/ARB use. Further, findings are limited to patients >5 years since in our experience patients < 5 years of age cannot undergo CMR without sedation. While it is possible, that 15 months follow-up was too short to detect therapeutic benefits, studies in other populations with preserved EF but measurable diastolic dysfunction have shown improvement in circumferential and longitudinal strain 12 months after ARB initiation [50].


These results support the need for rigorous, prospective clinical trials to identify more effective treatment regimens for DMD-associated cardiac disease.



Angiotensin Converting Enzyme Inhibitor


Angiotension Receptor Blocker


Beta blockers


beat per minute

Clinic Prior to CMR:

Previous Clinic Visit Documenting Medication and Dose Prior to CMR


Cardiovascular Magnetic Resonance


Duchenne Muscular Dystrophy


Circumferential Strain


Ejection Fraction

Group A:

Steroid only

Group B:

Steroid plus


heart rate


Left Ventricular


Left Ventricular Endiastolic Volume


Left Ventricular Mass




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Acknowledgements and Funding

This work is dedicated to the memory of William M. Gottliebson, MD. Supported in part by the Children's Heart Association of Cincinnati (WMG) and the National Institutes of Health HL069712 (DWB) Bethesda, MD.

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Correspondence to Kan N Hor.

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The authors declare that they have no competing interests.

Authors' contributions

KNH and DWB contributed to all aspects of the manuscript's conception, design, data analysis, collection, critical revision and final approval. HRA, contributed to statistical analysis, revision and final approval. MDT, LHC, JLJ, SVR, ESC, KJH, KW and WMG* contributed in interpretation of the data, critical revision and final approval of the manuscript. All authors have read and approved the final manuscript.

*WMG passed on September 17, 2010 and therefore, did not have the opportunity to give final approval of the manuscript but contributed significantly to it.

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Hor, K.N., Mazur, W., Taylor, M.D. et al. Effects of steroids and angiotensin converting enzyme inhibition on circumferential strain in boys with Duchenne muscular dystrophy: a cross-sectional and longitudinal study utilizing cardiovascular magnetic resonance. J Cardiovasc Magn Reson 13, 60 (2011).

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